Transcriptomic Analysis of Secondary Metabolite Biosynthesis in Solanaceae Medicinal Plants
Author(s) Name – PHS Dasanya, Madhavi Hewadikaram
Journal Name – Journal of Herbal Medicine
Publication Date – 2024/06/05
Abstract –
Introduction
Secondary metabolites produced in plants play a vital role in their adaptation to various biotic and abiotic stresses in the environment. Plant secondary metabolites possess significant value in the pharmaceutical and other herbal medicine-related industries. Therefore, the isolation and characterization of plant secondary metabolites have gained special interest in recent years. The elucidation of secondary metabolite biosynthetic pathways is required in the metabolic engineering of plant secondary metabolites in heterologous organisms.
Methods
Data were retrieved from various online electronic resources, including PubMed, Google Scholar, and Scopus, among others. Keywords such as Solanaceae plants, secondary metabolites, transcriptomic, medicinal plants, and RNA sequencing were used to formulate search strategies across approximately one hundred and eleven research and review articles.
Results
The outcomes of the de novo transcriptomic studies performed in Solanaceae medicinal plants have been extensively elucidated with a primary focus on the identification of candidate genes, pathway analysis differential gene expression analysis, the discovery of molecular markers, and the identification of transcription factors.
Discussions
The primary significance of de novo transcriptomic studies performed in Solanaceae medicinal plants has been discussed with significant limitations necessitating attention. Moreover, recent and future trends of de novo transcriptomic studies have been addressed with the intention of encouraging more de novo transcriptomic studies in Solanaceae medicinal plants.
DOI/Link to the Paper – https://doi.org/10.1016/j.hermed.2024.100899
Keywords – secondary metabolites, plant metabolic engineering, RNA sequencing, De novo assembly, functional annotation, Solanaceae family
Predicted roles of long non-coding RNAs in abiotic stress tolerance responses of plants
Author(s) Name – IUH Imaduwage, Madhavi Hewadikaram
Journal Name – Molecular Horticulture
Publication Date – 2024/5/15
Abstract –The plant genome exhibits a significant amount of transcriptional activity, with most of the resulting transcripts lacking protein-coding potential. Non-coding RNAs play a pivotal role in the development and regulatory processes in plants. Long non-coding RNAs (lncRNAs), which exceed 200 nucleotides, may play a significant role in enhancing plant resilience to various abiotic stresses, such as excessive heat, drought, cold, and salinity. In addition, the exogenous application of chemicals, such as abscisic acid and salicylic acid, can augment plant defense responses against abiotic stress. While how lncRNAs play a role in abiotic stress tolerance is relatively well-studied in model plants, this review provides a comprehensive overview of the current understanding of this function in horticultural crop plants. It also delves into the potential role of lncRNAs in chemical priming of plants in order to acquire abiotic stress tolerance, although many limitations exist in proving lncRNA functionality under such conditions.
DOI/Link to the Paper – https://doi.org/10.1186/s43897-024-00094-3
Keywords – Abiotic stress, Chemical treatment, Defense response, Long non-coding RNA, Non-coding RNA, Plant stress
Genetic polymorphisms of kappa-casein (CSN3) gene and its association with bovine milk properties.
Author(s) Name – Sharaz, M. S. M., and M. S. K. Rabindrakumar
Journal Name – Journal of Livestock Science
Publication Date – 2024/04/25
Abstract – Kappa-casein is an important subtype of casein protein which has many beneficial effects on the dairy industry as well as to the consumers’ health. This dual importance of kappa-casein underscores the interconnected relationship between the quality of milk and the sustainability of the dairy industry. Among the several factors, genes play a major role in influencing milk properties. In line with that, numerous genetic variants of the kappa casein gene have been identified and the two most prevalent variants, A and B, have shown significant associations with enhanced milk properties such as constituents, yield and technological properties. Hence, the review aims to provide an updated overview on the genetic polymorphisms of kappa-casein A and B variants in several bovine breeds from various parts of the world and their associations with milk properties particularly milk constituents, milk yield and technological properties such as rennet milk coagulation time and thermal resistance.
DOI/Link to the Paper – doi. 10.33259/JLivestSci.2024.150-159
Keywords – Kappa-casein; CSN3 gene; A and B variants; milk yield; milk constituents; rennet milk coagulation time.